Giovanna Barbieri
+39 091 6809537 | +39 091 6809529
giovanna.barbieri@irib.cnr.it

Institute
Istituto per la Ricerca e l’Innovazione Biomedica
Via Ugo La Malfa, 153 – 90146 Palermo
Tel. +39 0503757523
https://www.irib.cnr.it/

• MHC class II signalling and melanoma immune escape.
• Extracellular vesicles and immune escape of metastatic melanoma.
• Relationship between metastatic melanoma constitutively expressing MHC class II molecules and immune cells.
• MHC class II molecules are expressed in almost 50% of melanoma and the constitutive expression of MHC class II molecules in melanoma cells could be associated to metastatic progression and immune escape probably interfering with the effectiveness of checkpoint immunotherapy.

• M. Muller, J. Briscoe, C. Laxton, D. Guschin, A. Ziemiecki, O. Silvennoinen, A.G. Harpur, G. Barbieri, B.A. Witthuhn, C. Schindler, S. Pellegrini, A.F. Wilks, J.N. Ihle, G.R. Stark and I.M. Kerr. The protein tyrosine kinase JAK1 complements defects in interferon-a/b and -g signal transduction. Nature 366(6451), (1993) 129-135.

• C. Lutticken, U.M. Wegenka, J. Yuan, J. Buschmann, C. Schindler, A. Ziemiecki, A.G. Harpur, A.F. Wilks, K. Yasukawa, T. Taga, T. Kishimoto, G. Barbieri, S. Pellegrini, M. Sendtner, P.C. Heinrich and F. Horn. Association of transcription factor APRF and protein kinase JAK1 with the interleukin-6 signal transducer gp130. Science 263(5143), (1994) 89-92.

• N. Stahl, T.G. Boulton, T. Farruggella, N.Y. Ip, S. Davis, B.A. Witthuhn, F.W. Quelle, O. Silvennoinen, G. Barbieri, S. Pellegrini, J.N. Ihle and G.D. Yancopoulos.Association and activation of JAK-Tyk kinases by CNTF-LIF-OSM-IL6 b receptor components. Science 263(5143), (1994) 92-95.

• G. Barbieri, V. Deffrennes, T. Prod’homme, J. Vedrenne, F. Baton, C. Cortes, A. Fischer, M.R. Bono, B. Lisowska-Grospierre, D. Charron and C. Alcaide-Loridan. Isoforms of the class II transactivator protein. Int. Immunol. 14 (8), (2002) 839-848

• F. Costantini and G. Barbieri. The HLA-DR mediated signalling increases the migration and invasion of melanoma cells, the expression and lipid raft recruitment of adhesion receptors, PD-L1 and signal transduction proteins. Cell Signal. 36, (2017) 189-203. Epub 2017 May 8.
  

Ultracentrifuge for the isolation of lipid rafts and small extracellular vesicles; Odyssey infrared image system (LI-COR), for western blot analysis; Light optical microscopes and fluorescence microscopes, for immunofluorescence experiments, wound repair assay, cell migration and invasion assays; Cell cultures.